| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.184313 |
| Chromosome: | chromosome 16 |
| Location: | 4470385 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g670754 | (1 of 1) PTHR10217//PTHR10217:SF512 - VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGTGGGGGAGGGGGCTGTAGATACCCGC |
| Internal bar code: | CAGCCTTCTGAAGAACCGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 455 |
| LEAP-Seq percent confirming: | 98.2348 |
| LEAP-Seq n confirming: | 3840 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGCACACAGCCTTCAATA |
| Suggested primer 2: | GATCGGACTCACCGATGG |