| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.184323 |
| Chromosome: | chromosome 1 |
| Location: | 780732 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004050 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCCATTGCACAACGGCACAGTTTGGGCC |
| Internal bar code: | GCCTACGATAGCGTCAGATTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 26 |
| LEAP-Seq percent confirming: | 88.3657 |
| LEAP-Seq n confirming: | 319 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGCATAGCTATAAGCGGC |
| Suggested primer 2: | GGAGTACTCAGAAGCCGTCG |