| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184328 |
| Chromosome: | chromosome 6 |
| Location: | 7567355 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g300900 | GLE1 | Nucleoporin GLE1; (1 of 1) K18723 - nucleoporin GLE1 (GLE1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGCAGGCTCGGACAGCCGGAGGCTAAAG |
| Internal bar code: | TCAAGTGGTGAGTTTCGCGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 875 |
| LEAP-Seq percent confirming: | 98.9011 |
| LEAP-Seq n confirming: | 2160 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGCGAGGATTGCGAAGAG |
| Suggested primer 2: | TCCCAAAGTAAAGCATTGCC |