| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184329 |
| Chromosome: | chromosome 3 |
| Location: | 638013 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145767 | RRP6,RRP6a | putative exosome subunit; (1 of 1) K12951 - cation-transporting P-type ATPase D (ctpD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGTCGTGTTCCACACACTTCGCTCCTCA |
| Internal bar code: | TTAGCGTGGGTGCTAAGATGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 296 |
| LEAP-Seq percent confirming: | 99.5473 |
| LEAP-Seq n confirming: | 1979 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACCTCTCAACAGCCCTGC |
| Suggested primer 2: | CAGTAGTGGCTGCTGCTGAG |