| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.184335 |
| Chromosome: | chromosome 5 |
| Location: | 422330 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232750 | CNK4 | NimA-related protein kinase; (1 of 1) PTHR24361//PTHR24361:SF311 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCAAGAAACGAGTCATAGTACCGGATGA |
| Internal bar code: | AGTAGCCTCGGGAAATTGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 345 |
| LEAP-Seq percent confirming: | 99.322 |
| LEAP-Seq n confirming: | 293 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGATCTTACGAAACGTGG |
| Suggested primer 2: | TTCCTCCTCACCTGGTTGTC |