| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.184338 |
| Chromosome: | chromosome 1 |
| Location: | 2141250 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g011630 | (1 of 1) IPR001283//IPR002413//IPR003882//IPR014044 - Cysteine-rich secretory protein, allergen V5/Tpx-1-related // Ves allergen // Pistil-specific extensin-like protein // CAP domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATGAATAAACAGTCCCAGTACAAGACAC |
| Internal bar code: | GGTTGATGTTGGTACATCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1016 |
| LEAP-Seq percent confirming: | 96.8121 |
| LEAP-Seq n confirming: | 577 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGCTGCGTAATATCCA |
| Suggested primer 2: | GTGCCGTAACCACTAGCCAT |