| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.184368 |
| Chromosome: | chromosome 2 |
| Location: | 5146539 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105287 | (1 of 1) PF13821 - Domain of unknown function (DUF4187) (DUF4187) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAATACGAAGGACTCGTGCAGTCACACT |
| Internal bar code: | TATGCGAAGTGGGGCGCGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 781 |
| LEAP-Seq percent confirming: | 99.3902 |
| LEAP-Seq n confirming: | 978 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTAGAGGGGCGTTTAGGG |
| Suggested primer 2: | CCGAGTATTTCGGCTGTTGT |