| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184380 |
| Chromosome: | chromosome 2 |
| Location: | 1008127 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080350 | (1 of 1) K11836 - ubiquitin carboxyl-terminal hydrolase 5/13 [EC:3.4.19.12] (USP5_13, UBP14) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCACGCCCTGCCTGCCTGCCTACGGTT |
| Internal bar code: | GGGTATTACGGGTCGACCGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 61 |
| LEAP-Seq percent confirming: | 90.9836 |
| LEAP-Seq n confirming: | 111 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACCTGGGCTACCTCTACC |
| Suggested primer 2: | GGTGTCGTGTAGTAGGCCGT |