| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.184385 |
| Chromosome: | chromosome 6 |
| Location: | 6331738 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g291900 | PHD1 | (1 of 1) PTHR21148//PTHR21148:SF1 - PHOSDUCIN-RELATED // PHOSDUCIN-LIKE PROTEIN 3; Phosducin-like protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGTTCTTGGGACGTTGGACCGCAAGTCC |
| Internal bar code: | TTTCCCCCCGAGTTCTTAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 757 |
| LEAP-Seq percent confirming: | 99.6348 |
| LEAP-Seq n confirming: | 21824 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | GGCGGTGTGAACATAGACCT |