| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.184404 |
| Chromosome: | chromosome 7 |
| Location: | 3904239 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g339100 | (1 of 4) PTHR24361//PTHR24361:SF411 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGTGCGGGATTGTGCGGTATTTGCCTT |
| Internal bar code: | GTCGCTCGTGCATCAGCAACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 459 |
| LEAP-Seq percent confirming: | 98.8708 |
| LEAP-Seq n confirming: | 3590 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTGAAACAACACTGGA |
| Suggested primer 2: | AGTCCGAGTTTGCACCATCT |