| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.184410 |
| Chromosome: | chromosome 9 |
| Location: | 6074957 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g403219 | RPB5 | DNA-directed RNA polymerase II, 23 kDa polypeptide; (1 of 1) K03013 - DNA-directed RNA polymerases I, II, and III subunit RPABC1 (RPB5, POLR2E) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGACAGCATAGCGCCTCAGCCTGCAAA |
| Internal bar code: | GCGCGGGCAGGGTAAGTGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 588 |
| LEAP-Seq percent confirming: | 99.529 |
| LEAP-Seq n confirming: | 3381 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGTACAGTGCAGGAAGG |
| Suggested primer 2: | AAGGCGGTTAAATGCATGAC |