| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.184426 |
| Chromosome: | chromosome 12 |
| Location: | 142391 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g484350 | CKIN beta-gamma,SNRKBG1 | regulatory subunit beta-gamma of snRK1 complex in Chlamydomonas; (1 of 1) K07200 - 5'-AMP-activated protein kinase, regulatory gamma subunit (PRKAG) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAGCAGGCGGCCGCGGCACAGCAGCAGG |
| Internal bar code: | TGAAATGGTGCGCGCATCGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 389 |
| LEAP-Seq percent confirming: | 57.3529 |
| LEAP-Seq n confirming: | 1248 |
| LEAP-Seq n nonconfirming: | 928 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTGTATGGCGATTCGGAG |
| Suggested primer 2: | GAAGTCCGCAATCTTCTTGC |