| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184428 |
| Chromosome: | chromosome_1 |
| Location: | 2417975 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g013450 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCGTGGTCGTGATGCTTGTGCACGCCGTGG |
| Internal bar code: | CTTTTCAATAAACTCATCAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 830 |
| LEAP-Seq percent confirming: | 99.0147 |
| LEAP-Seq n confirming: | 6130 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGACAATTACGGCTCCGAC |
| Suggested primer 2: | AGAGAAATGCGATGAACGCT |