| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.184445 |
| Chromosome: | chromosome 6 |
| Location: | 1736986 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g261750 | BES3,RBMP1 | (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin); RuBisCO binding membrane protein 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCGCCGACTCGGCGTGCCACTTTGCAG |
| Internal bar code: | ACGCCGAGTTACGAGTGGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1049 |
| LEAP-Seq percent confirming: | 99.1301 |
| LEAP-Seq n confirming: | 4558 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGAAGAAGCAGGAGACG |
| Suggested primer 2: | GGGTAAGAGCAGGCACAGAG |