| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.184470 |
| Chromosome: | chromosome 13 |
| Location: | 3203634 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g585600 | (1 of 1) IPR011333//IPR019832 - POZ domain // Manganese/iron superoxide dismutase, C-terminal | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACCGGCAATGGTTGGGGTGGTCGTGGT |
| Internal bar code: | TGCTCGCAGCTTGACCTCCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1046 |
| LEAP-Seq percent confirming: | 99.7587 |
| LEAP-Seq n confirming: | 2067 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAGACTACAGCGTCAAGC |
| Suggested primer 2: | CCACACGACCACACATCTTC |