| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.184475 |
| Chromosome: | chromosome 17 |
| Location: | 254944 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g698000 | ATP2 | Mitochondrial F1F0 ATP synthase, beta subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACTGATCCCTCGAGTCCCAATCCGGTCA |
| Internal bar code: | GGGGGAATACGCTTTCCTTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 549 |
| LEAP-Seq percent confirming: | 99.8064 |
| LEAP-Seq n confirming: | 5154 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTCTTGCTTGACCTTCC |
| Suggested primer 2: | CCAAAACCCAGCATTCAGTT |