| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.184483 |
| Chromosome: | chromosome 8 |
| Location: | 751020 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g360550 | ERM3,NIRK,NIRK1 | Copper-containing nitrite reductase, mitochondrial; (1 of 1) 1.7.2.1 - Nitrite reductase (NO-forming) / Pseudomonas cytochrome oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCTTATCTTGATTGAGCATGCCACCCC |
| Internal bar code: | GCTCGGGACTGCGGGTCGTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 147 |
| LEAP-Seq percent confirming: | 54.3278 |
| LEAP-Seq n confirming: | 590 |
| LEAP-Seq n nonconfirming: | 496 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGTAGGGGAAGGAGTGGA |
| Suggested primer 2: | ACGCTGCTGACTCTATCGGT |