Insertion junction: LMJ.RY0402.184500_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.184500
Chromosome:chromosome 7
Location:2103439
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g326150 (1 of 34) IPR000571 - Zinc finger, CCCH-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCTTGCGTACACACACAGCGGCTTTGAG
Internal bar code:GAACCCTGGTTAACAGTGCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:978
LEAP-Seq percent confirming:99.5147
LEAP-Seq n confirming:3076
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGTTCAGAGACAACAAAA
Suggested primer 2:CCGACGACGACTATACGGTT