| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.184503 |
| Chromosome: | chromosome 14 |
| Location: | 3762864 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632350 | FAP138 | Coiled-Coil Flagellar Associated Protein 138 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCATAAACGGTTCTGCTGCCGTTTTCCT |
| Internal bar code: | GCCTGGGCGGAAAGCGAGGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1049 |
| LEAP-Seq percent confirming: | 99.5105 |
| LEAP-Seq n confirming: | 3456 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCAAGTCCCAAGTCAAT |
| Suggested primer 2: | AGGGACAGTTTTCCAACACG |