Insertion junction: LMJ.RY0402.184512_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.184512
Chromosome:chromosome 9
Location:6134236
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g405300 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCCAAGGCCATGCCAGCCCGCCTGCCTG
Internal bar code:TTATTTTTGGTGATCTGACCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:512
LEAP-Seq percent confirming:95.9184
LEAP-Seq n confirming:705
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGTAACTTGGGTTGCCTTCA
Suggested primer 2:GACTCGAGCCACTTCAGGTC