| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184532 |
| Chromosome: | chromosome 17 |
| Location: | 643607 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g700450 | GFY1 | (1 of 1) K07034 - uncharacterized protein (K07034); Putative acetate transporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTGAGGGAATCGTCGTTCCGGATTGTTT |
| Internal bar code: | CCAGGTTCGGCATCTTCAGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 570 |
| LEAP-Seq percent confirming: | 99.7016 |
| LEAP-Seq n confirming: | 7351 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACAGAACCTCTGCAACAA |
| Suggested primer 2: | TAGCGTTGTCTGAGGGCTTT |