| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.184538 |
| Chromosome: | chromosome 14 |
| Location: | 4117456 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g634113 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGTGCGCCGGGGCTGCTGCTGCTGCTCC |
| Internal bar code: | GACGTGTCCGAGCGGCTGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 691 |
| LEAP-Seq percent confirming: | 99.2472 |
| LEAP-Seq n confirming: | 791 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGGTTGAACTCAGGGCAG |
| Suggested primer 2: | GCAACCCCAACCTGAAGTTA |