Insertion junction: LMJ.RY0402.184539_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.184539
Chromosome:chromosome 9
Location:5774996
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g401960 (1 of 1) 2.8.2.1 - Aryl sulfotransferase / Sulfokinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGATTGCACACGTCCCACACACACACGG
Internal bar code:AAGAACCGGCCGTTGCGTTAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:843
LEAP-Seq percent confirming:99.7232
LEAP-Seq n confirming:1441
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCTGATAAGGTCGTGGGT
Suggested primer 2:CTTAGAGTCGAGGCCACCTG