Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.184539 |
Chromosome: | chromosome 9 |
Location: | 5775906 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g401960 | (1 of 1) 2.8.2.1 - Aryl sulfotransferase / Sulfokinase | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTAGAGTCGAGGCCACCTGCAGTGCTTG |
Internal bar code: | ACGCCAGGGTCTTCAGAACCAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 800 |
LEAP-Seq percent confirming: | 95.4118 |
LEAP-Seq n confirming: | 4159 |
LEAP-Seq n nonconfirming: | 200 |
LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTACCTACGCCTTCACGTC |
Suggested primer 2: | CAGTAGCATGAGACCTGCCA |