| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.184545 |
| Chromosome: | chromosome 7 |
| Location: | 4116151 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342900 | (1 of 2) 2.7.1.100 - S-methyl-5-thioribose kinase / MTR kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCGGTGGGATGCGCCGTATCGCATTTCG |
| Internal bar code: | CTACAACCGATATGCGTTGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1125 |
| LEAP-Seq percent confirming: | 88.4701 |
| LEAP-Seq n confirming: | 2770 |
| LEAP-Seq n nonconfirming: | 361 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTCAACGAAAGCACTGA |
| Suggested primer 2: | CTAACAGCCGGTACACCGAT |