| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.184547 |
| Chromosome: | chromosome 6 |
| Location: | 2414402 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g268300 | HTA9 | Histone H2A; (1 of 30) K11251 - histone H2A (H2A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCGGTGGTGACGTGCGGTCCCTTGTCA |
| Internal bar code: | GATGGGTCGAGTACGTTTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 489 |
| LEAP-Seq percent confirming: | 99.4293 |
| LEAP-Seq n confirming: | 4704 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGAGGAAGGTACAACGGA |
| Suggested primer 2: | CTGAAGAAGGGCAAGTACGC |