| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.184552 |
| Chromosome: | chromosome 9 |
| Location: | 2405656 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391650 | HCP4 | (1 of 4) 1.7.99.1 - Hydroxylamine reductase / Hydroxylamine (acceptor) reductase; Hybrid-cluster protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGCGAGGGCGTGGGGGAGGGGGGAGGGG |
| Internal bar code: | ATACAACGTAGGGTCGGCGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 710 |
| LEAP-Seq percent confirming: | 93.4313 |
| LEAP-Seq n confirming: | 1081 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTAGTAGCCCTCCCCCAA |
| Suggested primer 2: | AACCACTAGCATGGTTTGGC |