| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184574 |
| Chromosome: | chromosome 12 |
| Location: | 7487838 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556000 | ECP88 | 88 kDa extracellular polypeptide; (1 of 5) PF06742//PF06863 - Protein of unknown function (DUF1214) (DUF1214) // Protein of unknown function (DUF1254) (DUF1254) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGTTCTTGCTCGACTCGGGGGATTGGGC |
| Internal bar code: | CATTCGGGAGTCCTCATCAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1010 |
| LEAP-Seq percent confirming: | 99.3066 |
| LEAP-Seq n confirming: | 2005 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTTGCTGCTGCTGACACT |
| Suggested primer 2: | CTTACTCACGACTCGGCACA |