| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184587 |
| Chromosome: | chromosome 3 |
| Location: | 5026368 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g180650 | DEG7 | (1 of 2) PTHR22939//PTHR22939:SF66 - SERINE PROTEASE FAMILY S1C HTRA-RELATED // PRO-APOPTOTIC SERINE PROTEASE NMA111; Deg protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTACGGGTGGGCCATCTGGGTTGTCCTG |
| Internal bar code: | AGCGGGCATGCATGTCGAGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 221 |
| LEAP-Seq percent confirming: | 98.7928 |
| LEAP-Seq n confirming: | 15794 |
| LEAP-Seq n nonconfirming: | 193 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGCATTCCCCAATAAG |
| Suggested primer 2: | GTGGCCACTCATGAGGTTTT |