| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184588 |
| Chromosome: | chromosome 14 |
| Location: | 2736599 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g626900 | PMM1,PMM | Phophomannomutase; (1 of 1) K17497 - phosphomannomutase (PMM) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGACAGGGATCACGCAATAGCGCGGTTT |
| Internal bar code: | ACATACCTTTTGGTCAGTCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 782 |
| LEAP-Seq percent confirming: | 98.6383 |
| LEAP-Seq n confirming: | 5940 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCACACACACACACACA |
| Suggested primer 2: | CTACGGACACACATCATGGC |