| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.184588 |
| Chromosome: | chromosome_15 |
| Location: | 311658 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre15.g635700 | MAPKKK12 | Mitogen-Activated Protein Kinase Kinase Kinase | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTCTTCACACAGCAGCACCCGATGCCACCG |
| Internal bar code: | GCGGCAGGGGCGGTGGCAAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 186 |
| LEAP-Seq percent confirming: | 48.4018 |
| LEAP-Seq n confirming: | 636 |
| LEAP-Seq n nonconfirming: | 678 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGCAAGCAAGCAAAGATA |
| Suggested primer 2: | GACATGGAACAACAGCATGG |