| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184592 |
| Chromosome: | chromosome 12 |
| Location: | 2187961 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g508500 | (1 of 2) 3.4.17.4 - Gly-Xaa carboxypeptidase / Glycine carboxypeptidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCAGCTCCAGCACACGCGCATACACACA |
| Internal bar code: | TCCCTCAGCGCGCGCGCGCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 569 |
| LEAP-Seq percent confirming: | 99.6344 |
| LEAP-Seq n confirming: | 8449 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTCAAACTGGTGGAGGTG |
| Suggested primer 2: | TCTGAGTGCGTTTTGGACTG |