| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184595 |
| Chromosome: | chromosome_10 |
| Location: | 81412 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g418150 | FAP178 | Flagellar Associated Protein | sense | 5'UTR|outside_mRNA |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCTATTCAACTGGGGCCTTACAAAAACACA |
| Internal bar code: | ACTGTGGTCCGGGTATATCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 720 |
| LEAP-Seq percent confirming: | 99.6119 |
| LEAP-Seq n confirming: | 6417 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAAGCAGCAGAGCAAACGA |
| Suggested primer 2: | CTTACCGCCTGCAGTTCTTC |