Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.184601 |
Chromosome: | chromosome_16 |
Location: | 7720167 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g691440 | FAP43 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CGTGCTCAAGGACGGCGAGGACCGGTCCAG |
Internal bar code: | GCGTCACCGCGAACTCGTCTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 951 |
LEAP-Seq percent confirming: | 95.002 |
LEAP-Seq n confirming: | 2376 |
LEAP-Seq n nonconfirming: | 125 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCATGAAGGACTTTAAGCG |
Suggested primer 2: | TCTCGATGAAGGTGGGGTAG |