| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.184609 |
| Chromosome: | chromosome 9 |
| Location: | 2075376 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393950 | FHL2,FTSHI3,FTSHi3,FHL8 | (1 of 6) K03798 - cell division protease FtsH [EC:3.4.24.-] (ftsH, hflB); Putative chloroplast-import FtsH-like ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCGCCCAACCTTTTTTAGTCGCACTGT |
| Internal bar code: | GCAACCGGGTGTTCGCGGTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 189 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 510 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGGGATGGTGTGTATCA |
| Suggested primer 2: | TTGCTTCACTGGAGGTCCTT |