| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.184610 |
| Chromosome: | chromosome_14 |
| Location: | 882672 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g613550 | FAP203 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AACTGCTGTGCCCATCGAAAGCGGCTCCAC |
| Internal bar code: | GAAGACCGCGTTCACTCGTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1000 |
| LEAP-Seq percent confirming: | 99.0291 |
| LEAP-Seq n confirming: | 3468 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCAGACAGCACGATTGAA |
| Suggested primer 2: | GCTAGGTATGAAACGGCTGC |