Insertion junction: LMJ.RY0402.184628_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.184628
Chromosome:chromosome 3
Location:111407
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g143987 (1 of 1) IPR011016//IPR014048 - Zinc finger, RING-CH-type // Methylated-DNA-[protein]-cysteine S-methyltransferase, DNA binding intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTGTGTGAAACATGGTTGGCCAACCTTT
Internal bar code:TGTCCAACTGTTTGGCACCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:477
LEAP-Seq percent confirming:99.7046
LEAP-Seq n confirming:675
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCGAGAGAGTCAGATAGG
Suggested primer 2:CAGCTGCAGCACTTCTCAAC