| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184631 |
| Chromosome: | chromosome 9 |
| Location: | 5805401 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402108 | (1 of 1) PTHR13167//PTHR13167:SF25 - UNCHARACTERIZED // PIEZO, ISOFORM E; Piezo R-Ras-binding domain membrane protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGTTTCAGCACAACGCGCTGGTGCAATG |
| Internal bar code: | ACCGAACTCCGCAAGGTCTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 46 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 166 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTATGTCTGCTGCTGGT |
| Suggested primer 2: | GCACGCACGGTAAACCTTAT |