Insertion junction: LMJ.RY0402.184685_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.184685
Chromosome:chromosome_2
Location:8394739
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre02.g141606 DHC9 Flagellar inner arm dynein heavy chain, dynein c (monomeric) sense gene_edge/mRNA_edge/3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):TACTGTCTATTTCCCTGGTAAACAGTGTTG
Internal bar code:GCACTCCCGCCTATATAGGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:327
LEAP-Seq percent confirming:64.0036
LEAP-Seq n confirming:713
LEAP-Seq n nonconfirming:401
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GAGATCGCCAAGTTCTCCAG
Suggested primer 2:GTTGTCGTTGTTGTTGTCGG