| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184691 |
| Chromosome: | chromosome 10 |
| Location: | 4087595 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449700 | (1 of 7) PF00582 - Universal stress protein family (Usp) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCACGCAGCCTCGGCTTGCCATCGCCATC |
| Internal bar code: | CCTCGGGCATAATTGCTAGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 279 |
| LEAP-Seq percent confirming: | 99.6785 |
| LEAP-Seq n confirming: | 11783 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCACGATCACATTCTTCG |
| Suggested primer 2: | GGCCTACAGCTATGCGCTAC |