| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.184693 |
| Chromosome: | chromosome_12 |
| Location: | 5585083 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g531800 | FAP7 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AAGGCGACTAAACCTGCCAAAGCGTCCTGC |
| Internal bar code: | CGAGGGCAGCGTACCCGTGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 654 |
| LEAP-Seq percent confirming: | 98.8481 |
| LEAP-Seq n confirming: | 2746 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCATCGTACGCAACTCTC |
| Suggested primer 2: | TCAACGAGGTGTCAAACTGG |