| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.184699 |
| Chromosome: | chromosome 10 |
| Location: | 3309893 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443550 | (1 of 1) 2.7.11.11//2.7.11.12 - cAMP-dependent protein kinase / Protein kinase A // cGMP-dependent protein kinase / PKG II | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACCGCGCCTATCAGCCCCTGGCTTGCCG |
| Internal bar code: | GTCTTTGCCAGCCGCCGGACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 476 |
| LEAP-Seq percent confirming: | 98.8399 |
| LEAP-Seq n confirming: | 426 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCACGAACACGTCGTACT |
| Suggested primer 2: | AAAGCTTGAAGGATGGGCTT |