| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.184702 |
| Chromosome: | chromosome 10 |
| Location: | 291559 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419900 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGAGTTCAAATCTCACCCTCGTCAGTT |
| Internal bar code: | AGCCGGGTCTGGAGCGCAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 629 |
| LEAP-Seq percent confirming: | 99.4547 |
| LEAP-Seq n confirming: | 3465 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCGTTAGCGGTCAGGCTA |
| Suggested primer 2: | GCATGTTCGTCCAATCAATG |