| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.184708 |
| Chromosome: | chromosome 1 |
| Location: | 5023076 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034700 | (1 of 3) PF12843 - Putative quorum-sensing-regulated virulence factor (QSregVF_b) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGGTGCTCAGGAAGTTCTGCATGAACG |
| Internal bar code: | CTAACATGGCCTCAAGGCGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 622 |
| LEAP-Seq percent confirming: | 99.3691 |
| LEAP-Seq n confirming: | 315 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTGGCAATGGAGGTGTT |
| Suggested primer 2: | TAACGCACCGACACACAAAC |