| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.184711 |
| Chromosome: | chromosome 3 |
| Location: | 3938556 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171550 | SSA13 | cilia-sensing, structure and/or assembly; (1 of 1) PTHR21706//PTHR21706:SF15 - FAMILY NOT NAMED // TRANSMEMBRANE PROTEIN 65 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGATCGGGGCGAACGGTGTGGTGTGTGG |
| Internal bar code: | TAACACCCCCCATCGCTGGTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 99.721 |
| LEAP-Seq n confirming: | 7148 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAAGGTTCAGCATGTAGC |
| Suggested primer 2: | CCATGTACGCCTTTGTTCCT |