Insertion junction: LMJ.RY0402.184719_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.184719
Chromosome:chromosome 16
Location:2081469
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g657500 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCGAAAGTATTGTTTCCTGCGACGCAAC
Internal bar code:GGCAGACCCGGGTTCGAAAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:508
LEAP-Seq percent confirming:88.4058
LEAP-Seq n confirming:1769
LEAP-Seq n nonconfirming:232
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCAGGAGGTGTGAGGCTA
Suggested primer 2:GTGACTGAGTCCCTTGGTGG