| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184726 |
| Chromosome: | chromosome 12 |
| Location: | 4152474 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g518200 | PDI1,DNJ3 | (1 of 1) PTHR18929//PTHR18929:SF103//PTHR24078//PTHR24078:SF252 - PROTEIN DISULFIDE ISOMERASE // PROTEIN DISULFIDE-ISOMERASE A5 // DNAJ HOMOLOG SUBFAMILY C MEMBER // SUBFAMILY NOT NAMED; Protein disulfide isomerase, DnaJ-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGAACTGCCATGGTGTTGGAGTTACATT |
| Internal bar code: | AGACCGGTGCTCTTTGCGCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 923 |
| LEAP-Seq percent confirming: | 99.4842 |
| LEAP-Seq n confirming: | 3279 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGTAACCCAATCTGCCG |
| Suggested primer 2: | GGTCGTGGGTCTGAGTGATT |