| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.184759 |
| Chromosome: | chromosome 14 |
| Location: | 1972608 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g621351 | (1 of 2) 2.7.6.5 - GTP diphosphokinase / Stringent factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGGGGAGCGACGTGTGCGGGGTTGGAG |
| Internal bar code: | CTTGCGAGGGATAAGCTTCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 993 |
| LEAP-Seq percent confirming: | 99.67 |
| LEAP-Seq n confirming: | 1812 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGGACACGGTAACCCAGC |
| Suggested primer 2: | CCCAAACTAAACCAAAGCCA |