Insertion junction: LMJ.RY0402.184774_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.184774
Chromosome:chromosome 2
Location:6373969
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g115400 (1 of 2) IPR002889//IPR008979 - Carbohydrate-binding WSC // Galactose-binding domain-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACGCTGCCCGCAGACCCCTCTCAGCTGGC
Internal bar code:CTGGACATGTGCATCAACTTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1100
LEAP-Seq percent confirming:23.5294
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GAACATCGCGTACTTGAGCA
Suggested primer 2:CATCAACAAGTCCGAGCTGA