| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.184792 |
| Chromosome: | chromosome 15 |
| Location: | 1112320 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g638000 | CLR9,RAP8,OPR69 | RAP-domain containing protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGATCGGTTTCTGCCAGGTATTGCTTGG |
| Internal bar code: | GACTGTTTAAAGTCGTGAGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 994 |
| LEAP-Seq percent confirming: | 99.8562 |
| LEAP-Seq n confirming: | 8334 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCTGCTATCAGGCGCTTC |
| Suggested primer 2: | AGGTCAGGAGGGAACCATCT |